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A method for the estimation of dietary and microbial protein in duodenal digesta of ruminants


, : A method for the estimation of dietary and microbial protein in duodenal digesta of ruminants. British Journal of Nutrition 32(1): 99-112

A method is described for estimating microbial and dietary protein entering the small intestine of sheep, based on an intraruminal infusion of sulphate-35S and isolation of the labelled microbial protein. Samples of the microbial fraction contained in duodenal digesta were isolated by high-speed centrifuge and specific activity of methionine was estimated in the microbial fraction and duodenal digesta.

US$19.90

PMID: 4846158

DOI: 10.1079/bjn19740061


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