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Accelerated chromatographic method for determination of hydroxyproline

, : Accelerated chromatographic method for determination of hydroxyproline. Analytical Biochemistry 49(1): 155-163

Hydroxyproline in urine was estimated automatically by chromatography on spherical sulphonated polystyrene resin, PA-35, and reaction with ninhydrin. Each analysis took 30 min and reproducibility and recovery were >98%. Eight subjects given for 4 days 283 g meat daily as the source of collagen excreted on average 38.7 mg hydroxyproline/24 h.


PMID: 5081096

DOI: 10.1016/0003-2697(72)90253-9

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