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Accelerated complement fixation reaction in foot-and-mouth disease


, : Accelerated complement fixation reaction in foot-and-mouth disease. Veterinariya, Moscow (9): 110-112

The test was designed for diagnostic examination of field specimens against six different FMD antisera, using a spectrophotometer to evaluate the results. After addition of the haemolytic system, the tubes were incubated at 38 deg until cloudiness appeared in the first 2 or 3 tubes. Haemolysis was then interrupted by centrifugation at 800-1000 g for 7-10 minutes, and the supernatant fluid was examined in the spectrophotometer for haemoglobin content. A result was obtainable within 45 minutes.

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