+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Acetylcholinesterase secretion by parasitic nematodes. I. Evidence for secretion of the enzyme by a number of species

, : Acetylcholinesterase secretion by parasitic nematodes. I. Evidence for secretion of the enzyme by a number of species. International Journal for Parasitology 3(5): 589-597

Studies made on the acetylcholinesterase of the parasitic stages of Trichostrongylus colubriformis, T. axei, Oesophagostomum venulosum, Ostertagia circumcincta, Chabertia ovina and Haemonchus contortus from sheep, Oesophagostomum radiatum, Cooperia pectinata and H. placei from calves, Nippostrongylus brasiliensis from rat and Nematospiroides dubius from mouse revealed levels ranging from 80 and 84 mu moles of acetylthiocholine hydrolysed/g/hour for H. placei and C. pectinata adults, respectively, to 22,976 mu moles/g/hour for 4th-stage T. colubriformis. Little of the activity was due to pseudocholinesterase, and 41 to 64% of the enzyme was soluble except for C. pectinata, H. contortus adults and all the infective larvae studied, which all had less than 20% soluble enzyme. T. colubriformis, T. axei, O. radiatum and O. venulosum had high total levels of acetylocholinesterase, much of it soluble and apparently located in the oesophageal and/or excretory glands, and showed high rates of enzyme release in vitro; Ostertagia circumcincta and Chabertia ovina showed moderate rates of enzyme release, and Cooperia pectinata very low rates. A study of the kinetics of inhibition of acetylcholinesterase activity in the soluble extract of Nippostrongylus brasiliensis using coroxon, together with acrylamide gel electrophoresis, provided evidence for 2 different types of the enzyme differing in sensitivity to the inhibitor.


PMID: 4795374

Other references

Dascal N.; Boton R., 1990: Interaction between injected calcium ion and intracellular calcium ion stores in xenopus oocytes. Febs Letters 267(1-2): 22-24

Szegedy-Maszak, M., 2002: When does a child need help?. U.S. News & World Report 133(18): 74-74

Garay, J.; Correa, P.; Bravo, J.C.; Schneider, B.G., 1999: Microsatellite instability and loss of heterozygosity in gastric intestinal metaplasia. Modern Pathology 12(1): 75A, Jan

Engstrom, D.F.; Mathison, G.W., 1988: Effects of beta -glucans and other factors on the digestion and utilization of barley by ruminants. Six lots of barley containing from 3.5 to 4.8% beta -glucans were given to 120 steers for 117 days. Barley type and processing method (steam or dry rolling) had no effect on weight gain, feed conversion efficiency or carcass characteristics. A cha...

Henning, P.; Conaway, H.Herschel.; Lerner, U.H., 2015: Retinoid receptors in bone and their role in bone remodeling. Vitamin A (retinol) is a necessary and important constituent of the body which is provided by food intake of retinyl esters and carotenoids. Vitamin A is known best for being important for vision, but in addition to the eye, vitamin A is necessary...

Gabbrielli, S., 1983: Role of the microenvironment in determining the typology of pulmonary carcinoma. Archivio "de Vecchi" Per L'anatomia Patologica E la Medicina Clinica 65(2-3): 239-250

D.F.ancesco, L.; Berlingieri, D.; Lama, G., 1973: On serum immunoglobulins in full-term newborn infants and their mothers. La Pediatria 81(3): 415-425

Zacharieva, S.; Andreeva, M.; Orbetzova, M.; Wippermann, M.; Mucha, I.; Andonova, K.; Sheitanova, S., 1996: Effects of corticotropin-releasing hormone on ACTH, cortisol and 13, 14-dihydro-15-keto prostaglandin E2 in patients with diabetes insipidus before and after captopril treatment. A corticotropin-releasing hormone (CRH) test was performed on 7 patients with central diabetes insipidus (DI) and on 7 healthy subjects. The test was repeated on the patients with DI after 3 days of oral treatment with captopril at a dose of 100 m...

Xue Lu; Jiang TieMin; Ren FaZheng; Chen LiJun, 2006: Study on the milk-clotting mechanism of glutinous rice wine. The milk clotting mechanism of glutinous rice wine was studied. Results showed that milk clotting was caused by the protease secreted by the microorganism in the glutinous rice wine.

Salerno, A., 1964: Serum protein polymorphism in bovines. A genetic and biochemical study of post-albumins in the Romagna breed. The investigations were carried out on the same cattle as in No. 201. Three phenotypes were identified, AA, AB and BB, determined by 2 co-dominant alleles P alpha A and P alpha B. The frequency of these alleles was 0.1287 and 0.8713 resp. in the p...