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Cryopreservation of pathogenic African trypanosomes in situ: metacyclic and bloodstream forms

, : Cryopreservation of pathogenic African trypanosomes in situ: metacyclic and bloodstream forms. Journal of Protozoology 19(3): 494-497

The most satisfactory of several methods for cryopreservation of Trypanosoma vivax, T. Congolense and T. brucei metacyclic forms in tsetse fly organs, as well as bloodstream forms in host blood, was as follows: infected fly proboscises and salivary glands were placed in glass capillary tubes containing foetal calf serum with 8% (v/v) glycerol as the cryoprotectant. The method for bloodstream forms involved the addition of glycerol directly to infected blood, which was then dispensed into capillary tubes. Next the tubes were placed in paper containers inside a glass test tube with a 5mm thick plasticine jacket. The insulated assembly was suspended in the liquid nitrogen vapour phase in a refrigerator for 45 minutes. Under these conditions, the cooling rate was 2 deg C/min. The frozen samples were transferred to permanent storage. The viability and infectivity of the preserved organisms were satisfactory upon testing, and no antigenic changes were observed. Laboratory and field applications of the method are discussed.


PMID: 5074424

DOI: 10.1111/j.1550-7408.1972.tb03511.x

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