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Isolation and fusion of protoplasts from sugarcane young leaves

, : Isolation and fusion of protoplasts from sugarcane young leaves. Haploids in higher plants Advances and potential II Methods of producing haploids: 141-142

There were significant differences in protoplast numbers released from young leaves of five Saccharum species, including S. officinarum, S. spontaneum, S. sinense, S. robustum and Saccharum interspecific hybrids, using a combination of the two commercial enzymes, Onozuka and Macerozyme, S. robustum tending to give the highest output. Viable protoplasts were produced in osmotic concentrations ranging from 0.3-0.9 M, but the highest yield occurred at 0.6-0.7 M.


Other references

Friedman, M.W., 1955: Congenital symblepharon. American Journal of Ophthalmology 39(2 Pt 1): 236-237

Anonymous, 1886: Origin Of Fat In Animals. Science 7(171s): 444-446

Hoffmann, L., 1990: Continuous measuring of heat production during the course of the day in fully grown rats at different nutrition levels and different environmental temperatures. The heat production of 4 rats was measured by means of indirect calorimetry over 20 h/d at intervals of 4 min at ambient temperatures of 30, 25, 20 and 15 degrees C and feed intakes of 0, 4, 8 and 12 g/d. When the rats were hungry, their heat prod...

Justová, V.; Stárka, L.; Wilczek, H.; Pacovský, V., 1976: A simple radioassay for 25-hydroxycholecalciferol without chromatography. A simple method to assay 25-hydroxycholecalciferol in human plasma without chromatography is described. The method includes ammonium sulphate precipitation of 25-hydroxycholecalciferol bound to plasma-transport globulins as a purification step, ex...

Mancinelli A.L., 1988: Some thoughts about the use of predicted values of the state of phytochrome in plant photomorphogenesis research. Predicted values of photoequilibrium ratios and rates of photoconversion and cyling, calculated from know optical parameters of purified phytochrome and the spectral photon flux distribution of the light sources used, are often applied in the eval...

Pinkerton, J.V., 2008: An expert offers advice on the use of unregulated, custom-compounded hormones. Geriatrics 63(9): 1 P Following 20-1 P Following 20

Hoshina, Hideto., 2000: New record of Anisotoma besucheti (Coleoptera, Leiodidae) from Kyushu, Japan. Elytra. Nov 15; 282: 304

Cardoso, MA., 1973: Nova contribuYao para o estudo dos colembolos de MoYambique. Revista cienc biol (Ser A): 6: 7-19

Bohle, Hw, 1972: Comparative studies on the early larval case construction of the brachycentrid species Micrasema minimum MacLachlan and Brachycentrus montanus Klapalek (Trichoptera, Insecta). Zoologische Jahrbeucher: Abteilung feur Systematik Okologie und Geographie der Tiere: 9 (4) 507-544

Herve Michel, 1989: Preliminary geochemical results from intrusions at the SE border of the Lac St-Jean anorthosite complex, Quebec. Program with Abstracts - Geological Association of Canada(Mineralogical Association of Canada(Canadian Geophysical Union, Joint Annual Meeting 14: 51