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Isolation and fusion of protoplasts from sugarcane young leaves


, : Isolation and fusion of protoplasts from sugarcane young leaves. Haploids in higher plants Advances and potential II Methods of producing haploids: 141-142

There were significant differences in protoplast numbers released from young leaves of five Saccharum species, including S. officinarum, S. spontaneum, S. sinense, S. robustum and Saccharum interspecific hybrids, using a combination of the two commercial enzymes, Onozuka and Macerozyme, S. robustum tending to give the highest output. Viable protoplasts were produced in osmotic concentrations ranging from 0.3-0.9 M, but the highest yield occurred at 0.6-0.7 M.

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