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Isolation of rabies virus from saliva in cell culture.


, : Isolation of rabies virus from saliva in cell culture.. Tecnica Pecuaria en Mexico (18): 89-92

Vampire bat virus was injected intracerebrally into 3-week-old mice. Saliva was taken whem symptons were noted. Three swabs soaked in culture medium were used for each mouse. One swab was seeded onto BHK monolayer after an interval of one hour, and the other two after 24 or 48 hours at -70 deg C. Isolation of the virus from saliva was confirmed by the fluorescent antibody test in 13 of 14 mice. One case was positive only after 24 hours' refrigeration and 96 hours' incubation.

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