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Studies on the polyhedrosis virus forming polyhedra in the midgut-cell nucleus of the silkworm, Bombyx mori. I. Purification procedure and form of the virion


, : Studies on the polyhedrosis virus forming polyhedra in the midgut-cell nucleus of the silkworm, Bombyx mori. I. Purification procedure and form of the virion. Journal of Invertebrate Pathology 22(2): 266-272

A procedure for the isolation and purification of a new polyhedrosis virus that forms polyhedra in the midgut-cell nucleus of larvae of Bombyx mori (L.) is described. The method is a modification of that used by Y. Hayashi & F. T. Bird (1970) for the isolation of free cytoplasmic-polyhedrosis virus (CPV). The purified virions were spherical in shape with several projections measuring 62 nm in diameter. The sedimentation coefficient of this virion was 430 S.

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DOI: 10.1016/0022-2011(73)90144-4


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