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Studies on the preparation of insoluble derivatives of rennin (coupling to Sepharose and aminoethylcellulose)


, : Studies on the preparation of insoluble derivatives of rennin (coupling to Sepharose and aminoethylcellulose). Japanese Journal of Dairy Science 23(3): A83-A87

Insoluble derivatives of rennin were prepared as described by Green & Crutchfield By coupling 100 mg rennin to 200 mg Sepharose at pH 6.3, 160-190 mg insoluble products were yielded; they contained 20-26% rennin and showed 10-12% of the activity of native rennin. By coupling 50 mg rennin to 1 g aminoethylcellulose at pH 6.0, 850-870 mg products containing 3.5% rennin were yielded and showing 9-13% of the activity of native rennin.

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