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Studies on the release of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) from boar and ram spermatozoa


, : Studies on the release of glutamic oxaloacetic transaminase (GOT) and glutamic pyruvic transaminase (GPT) from boar and ram spermatozoa. Veterinaria Italiana 25(1/2): 3-21

Samples of ram semen (18 ejaculates) were diluted 1:10 in 2.9% sodium citrate solution. After centrifugation, GOT and GPT activity were estimated in the supernatant fluid. Estimates were made just after semen collection and on samples kept for 2 h at 37 deg C. The sperm-rich fraction of boar ejaculates was diluted 1:10 in Beltsville F3 extender without glycerol and with 5% glycerol. Samples without glycerol were tested immediately and those with glycerol were tested after 4 h at room temp.

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