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Studies on the significance of some isoenzymes of the alkaline phosphatase in the biological value of bull's spermatozoa


, : Studies on the significance of some isoenzymes of the alkaline phosphatase in the biological value of bull's spermatozoa. Comptes Rendus de l' Academie Agricole Georgi Dimitrov 7(1): 79-80

12 ejaculates were centrifuged at 6000 r.p.m., and separated spermatozoa were suspended in a saline solution, homogenised and centrifuged at 5000 r.p.m. for 15 min. The supernatant liquid was examined electrophoretically. Three isozymes were found in 8 samples and 4 isozymes in 4 samples. The isozymes 1 and 4 were fast, and the isozymes 2 and 3 were slow.

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