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Assembly of a spherical plant virus


, : Assembly of a spherical plant virus. Philosophical Transactions of the Royal Society, B 276(943): 113-122

The previously described (Adolf & Butler, Nature, UK 255: 737, 1975) reassembly of cowpea chlorotic mottle virus in vitro involved the co-aggregation of the RNA and protein around neutrality and at ionic strength 0.2, giving yields of 70% encapsidation at pH 6. The reaction was independent of temp. at 5-25 deg C and did not require the presence of Mg2+ ions. The reassembled virus showed a stability similar to that of native CCMV, with the same change in sedimentation coefficient around pH 6.5.

US$19.90

PMID: 13422

DOI: 10.1098/rstb.1976.0102


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