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Cucumber mosaic virus-associated RNA 5 III. Little nucleotide sequence homology between CARNA 5 and helper RNA

, : Cucumber mosaic virus-associated RNA 5 III. Little nucleotide sequence homology between CARNA 5 and helper RNA. Virology 80(1): 204-213

RNase resistant nucleic acid structures were isolated from plants infected with the S str. of CMV. The behaviour of these nucleic acids in relation to the corresponding single stranded (ss) RNAs in rate zonal centrifugation experiments and/or in polyacrylamide gel electrophoresis suggested that they are the double stranded (ds) RNAs of CMV and CMV-associated RNA 5 (CARNA 5). The yields of dsRNAs isolated from different host plants infected with CMV varied significantly. In host plant-inoculum combinations producing large amounts of CARNA 5, the dsRNAs consisted of 90% or more or dsCARNA 5, while yields of dsRNAs were X2 - 5 higher than in the absence of dsCARNA 5. Separation of dsCARNA 5 from the viral dsRNAs was accomplished by rate zonal centrifugation on sucrose gradients, and resulted in a gel-electrophoretically pure product. This material hybridized with 3H-labelled CARNA 5. In competition hybridization experiments only 9% of the radioactivity of [3H]CARNA 5 would not have hybridized at infinitely high conc. of CMV RNA 1 + 2 + 3, and none with CMV RNA 4. Thus CARNA 5 has no nucleotide sequence homology with CMV RNA 4, and, at most, only 0.1 of its nucleotide sequence occurs in the genomic RNAs of CMV.


PMID: 878313

DOI: 10.1016/0042-6822(77)90393-2

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