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Cucumber mosaic virus-associated RNA 5 III. Little nucleotide sequence homology between CARNA 5 and helper RNA


, : Cucumber mosaic virus-associated RNA 5 III. Little nucleotide sequence homology between CARNA 5 and helper RNA. Virology 80(1): 204-213

RNase resistant nucleic acid structures were isolated from plants infected with the S str. of CMV. The behaviour of these nucleic acids in relation to the corresponding single stranded (ss) RNAs in rate zonal centrifugation experiments and/or in polyacrylamide gel electrophoresis suggested that they are the double stranded (ds) RNAs of CMV and CMV-associated RNA 5 (CARNA 5). The yields of dsRNAs isolated from different host plants infected with CMV varied significantly. In host plant-inoculum combinations producing large amounts of CARNA 5, the dsRNAs consisted of 90% or more or dsCARNA 5, while yields of dsRNAs were X2 - 5 higher than in the absence of dsCARNA 5. Separation of dsCARNA 5 from the viral dsRNAs was accomplished by rate zonal centrifugation on sucrose gradients, and resulted in a gel-electrophoretically pure product. This material hybridized with 3H-labelled CARNA 5. In competition hybridization experiments only 9% of the radioactivity of [3H]CARNA 5 would not have hybridized at infinitely high conc. of CMV RNA 1 + 2 + 3, and none with CMV RNA 4. Thus CARNA 5 has no nucleotide sequence homology with CMV RNA 4, and, at most, only 0.1 of its nucleotide sequence occurs in the genomic RNAs of CMV.

US$19.90

PMID: 878313

DOI: 10.1016/0042-6822(77)90393-2


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Veltmann, C.; Lohmaier, S.; Schlosser, T.; Shai, S.; Ehlgen, A.; Pohl, C.; Becher, H.; Tiemann, K., 2002: On the design of a capillary flow phantom for the evaluation of ultrasound contrast agents at very low flow velocities. Recently, a new imaging technology has become available that allows the evaluation of tissue perfusion using echo-contrast agents in real-time imaging: power pulse inversion imaging (PPI). Although numerous in vitro phantoms have been designed for...

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Banakh O.S.; Datsenko I.I.; Maslyakevich Y.V.; Tymkevich A.Z., 1985: Influence of chemical processing on chromatographic properties of natural zeolites from Transcarpathia. Pages 165-167 1985

Comeau, A.Marie.; Accurso, F.J.; White, T.B.; Campbell, P.W.; Hoffman, G.; Parad, R.B.; Wilfond, B.S.; Rosenfeld, M.; Sontag, M.K.; Massie, J.; Farrell, P.M.; O'Sullivan, B.P., 2007: Guidelines for implementation of cystic fibrosis newborn screening programs: Cystic Fibrosis Foundation workshop report. Newborn screening for cystic fibrosis offers the opportunity for early intervention and improved outcomes. This summary, resulting from a workshop sponsored by the Cystic Fibrosis Foundation to facilitate implementation of widespread high quality...

Lin Li. et al., 1993: A new record of Haliotrema on the gills of Sparus macrocephalus Basilewsky from China. Annual Bulletin of the Society of Parasitology Guangdong Province. December; 14-15: 312

Luck, H., 1981: The low temperature storage of milk on farms and in factories and its influence on cheese quality. A recent survey of 200 samples each of refrigerated milk from farm tanks and road tankers showed mean total bacterial counts of 150 000 and 296 000/ml, psychrotrophic counts of 14 900 and 58 000/ml, coliform counts of 540 and 2200/ml and thermodur...

Skeats, E.W., 1905: On the chemical and mineralogical evidence as to the origin of the Dolomites of Southern Tyrol. Quarterly Journal of the Geological Society, lxi 97-141

Qu, J.; Zhang, C.M.o; Zhai, Z.H., 1995: In vitro nuclear reassembly using Xenopus sperm chromatin and egg extracts. Cell-free extracts prepared from activated eggs of Xenopus laevis were able to be used in assembling nuclei surrounded by demembranated Xenopus sperm chromatin. When the sperm chromatin which has been demembranated by lysolecithin was incubated in...

Wang H H., 1977: The probable nuclear ratio of the mycelium of commercial cultivated mushroom agaricus bisporus grown on plate cultures. Chiu, Ren-Jong Et Al (Ed ) Mushroom Science, Ix Part 11 Proceedings Of The Ixth International Scientific Congress on The Cultivation Of Edible Fungi Taipei, Taiwan, Nov 15-18, 1974 Viii+271p Illus Chinese Society For Horticultural Science: Taipei, Taiwan 27-35

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