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Galactosyltransferase: confirmation of equilibrium-ordered mechanism

, : Galactosyltransferase: confirmation of equilibrium-ordered mechanism. Biochemical and Biophysical Research Communications 66(1): 139-143

MnCl2 purified with diphenylthiocarbazone and used as a substrate for bovine milk galactosyltransferase resulted in kinetic patterns indicative of an equilibrium-ordered mechanism for the addition of Mn2+. The use of analytical reagent MnCl2 without purification produced kinetic plots intersecting to the left of the vertical axis, indicating sequential addition of Mn2+.


PMID: 1164419

DOI: 10.1016/s0006-291x(75)80305-6

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