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Isolation and properties of IgG-inducing antigens from Dirofilaria immitis

, : Isolation and properties of IgG-inducing antigens from Dirofilaria immitis. Japanese Journal of Parasitology 26(1): 17-24

Antigens present in a crude extract of Dirofilaria immitis which induced IgG in rats, were partially purified and separated from allergen (IgE-inducing antigen) by a combination of DEAE-Sephadex A-50 chromatography and Sephadex G-200 gel filtration. The molecular weight of the purified preparation (Frac. D-a or E-a) was estimated to be approximately 600,000 by gel filtration. It was resistant to tryptic digestion and periodate oxidation but very sensitive to pronase digestion.


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