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Isolation and purification of alpha -lactalbumin - a component of lactose synthetase


, : Isolation and purification of alpha -lactalbumin - a component of lactose synthetase. Biokhimiya 40(3): 584-587

The authors describe a simplified method developed for isolation and purification of alpha -lactalbumin by fractionation with (NH4)2SO4 and gel filtration through Sephadex G-50. Electrophoresis showed that the fraction obtained contained virtually no other proteins. Recovery of alpha -lactalbumin in the final stage of purification was 30%.

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