geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix


, : Isolation of chick intestinal cells infected with second-generation schizonts of Eimeria necatrix. Parasitology 74(1): 19-26

After infection with E. necatrix, chick intestinal cells undergo a profound increase in size. Methods are described for isolating and purifying E. necatrix infected cells containing second-generation schizonts at various times after infection. The purification procedures depended upon three fundamental properties of infected cells: namely (1) large size, (2) resistance to shearing and (3) decreased susceptibility to osmotic shock in hypotonic solutions.

US$19.90

PMID: 840495

DOI: 10.1017/s0031182000047491


Other references

Bulatov, OA., 1989: The role of environmental factors in fluctuations of stocks of walleye pollock (Theragra chalcogramma) in the eastern Bering Sea. Canadian Special Publication of Fisheries and Aquatic Sciences, 108: 353-357

Popivanova, P.; Ivanov, S.; Mazneĭkova, V.; Stoĭkova, V.; Tsekova, K., 2002: Retrospective study of the pregnancy course in preeclamptic patients. 105 women had been evaluated using a retrospective analysis for the period 1999-2000. Clinical and laboratory data indicated development of preeclampsia and the pregnancy progress, antenatal complications, mode of delivery and perinatal outlet has...

Takahashi, T.; Shimizu, H.; Akagi, R.; Morita, K.; Sassa, S., 2006: Heme oxygenase-1: A new drug target in oxidative tissue injuries in critically ill conditions. Oxidative stresses associated with ischemia/reperfusion, neutrophil activation, and anesthesia with certain volatile agents, etc., are thought to play an important role in the development of acute organ failure in critical illnesses, such as acute...

Wet, P.V.D.M. de; Groenewald, J.A.; Boshoff, A.B., 1992: Managerial behaviour characteristics and financial success or failure in farming - an exploratory study. This paper studies the relationship between managerial action and farm financial success of a sample of 10 farmer clients of a commercial bank in the Northern Transvaal of South Africa, using data for 1980-85. Farmers who were financially successf...

Seo K., 1983: Synthesis of 1 2 4 tri o acetyl 6 deoxy 6 c isopropylphosphinyl 3 5 di o methyl d gluco septanose and 1 2 4 tri o acetyl 3 o benzyl 6 c butylphosphinyl 6 deoxy 5 o methyl d gluco septanose/. The Michaelis-Arbuzov reaction of 6-deoxy-6-iodo-1,2-O-isopropylidene-3-O-methyl-.alpha.-D-glucofuranose with diethyl isopropylphosphonite afforded 6-deoxy-6-C-(ethoxyisopropylphosphinyl)-1,2-O-isopropylidene-3-O-methyl-.alpha.-D-glucofuranose (3)...

Takada, S.; Magira, T.; Yamamura, M., 1989: Alteration of DNA primase activity by phosphorylation and de-phosphorylation of histone H1. To investigate the effect of histone H1 on DNA primase activity, partially purified DNA primase from mouse FM3A cells was used. It was found that histone H1 dose dependently inhibited DNA primase. Interestingly phosphorylation of histone H1 reduce...

Adldinger, H.K.; Calnek, B.W., 1971: An improved in vitro assay for cell-free Marek's disease virus. Brief report. Archiv für die Gesamte Virusforschung 34(4): 391-395

Levesque, H.M.; Scaffidi, D.; Polkinghorne, C.N.; Sorensen, P.W., 2011: A multi-component species identifying pheromone in the goldfish. Although it has been established that sexually-immature goldfish and their relatives recognize members of their own species by using chemicals that they release, the identity of this cue(s) and whether it might be produced and used by other life s...

Wang, M-Xiang., 2015: Exploring tertiary enamides as versatile synthons in organic synthesis. Tertiary enamides have long been thought of as stable and marginally valuable enamine variants in synthesis. This notion has been challenged, however, in recent years. Enabling the regulation of the cross-conjugation system of the tertiary enamide...

Rani, M.; Prakash, D.; Sobti, R.C.; Jain, R.K., 1996: Plasmid-mediated degradation of o-phthalate and salicylate by a Moraxella sp. A Moraxella sp. strain VG45 capable of utilizing o-phthalate and salicylate as a sole source of carbon and energy was isolated. The degradation of o-phthalate occurs via phthalate 4,5-dioxygenase, 4,5-dihydro-4,5-dihydroxyphthalate dehydrogenase,...