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Isolation of envelope membranes from bundle sheath chloroplasts of maize

, : Isolation of envelope membranes from bundle sheath chloroplasts of maize. Plant Physiology 60(5): 767-770

Bundle sheath strands were isolated from maize leaves treated with preparations of cellulase, hemicellulase and pectinase. A 3-phase discontinuous gradient yielded 2 fractions of envolope membranes from bundle sheath chloroplasts; buoyant densities were 1.06 and 1.09 g/cm3. The lighter fraction contained membrane vesicles under light microscopy, but centrifugation produced a pellet that was too small and unstable for purposes of electron microscopy.


PMID: 16660181

DOI: 10.1104/pp.60.5.767

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