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Penetration of hamster and rabbit zona-free eggs by rat and mouse spermatozoa with special reference to sperm capacitation

, : Penetration of hamster and rabbit zona-free eggs by rat and mouse spermatozoa with special reference to sperm capacitation. Journal of Reproduction and Fertility 46(1): 239-241

Eggs recovered from superovulated golden hamsters and rabbits were washed with 0.1% hyaluronidase to remove the cumulus oophorus and with 0.01% (hamsters) or 0.1% (rabbits) trypsin to remove the zona pellucida. The denuded eggs were added to suspensions of spermatozoa from nature Sprague-Dawley rats or mice and were cultured. Penetration of hamster eggs increased gradually with time after exposure to freshly prepared mouse sperm suspensions.


PMID: 1271344

DOI: 10.1530/jrf.0.0460239

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