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Purification of canine prolactin by preparative isotachophoresis

, : Purification of canine prolactin by preparative isotachophoresis. Journal of Endocrinology 69(1): 127-132

Preparative isotachophoresis in polyacrylamide gel using carrier ampholytes as 'spacers' has been used to purify prolactin from canine pituitary extracts. Using ampholine pH 5-8 as spacers, a prolactin fraction was obtained which was essentially homogeneous as judged by the criteria of disk electrophoresis and isoelectric focusing.


PMID: 5569

DOI: 10.1677/joe.0.0690127

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