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Fluorometric determination of thiamine and its mono-, di-, and triphosphate esters

, : Fluorometric determination of thiamine and its mono-, di-, and triphosphate esters. Methods in Enzymology 62: 58-59

In a modification of the method of Lewin and Wei (NAR 37, 2428) a 5 mu l-sample is shaken with 3 ml 50% ethanol then for 2 min with 0.5 ml mixture of 15 ml 15% NaOH and 1 ml 2% K3Fe(CN)6. Fluorescence is measured after adding 10 mu l 30% H2O2.


PMID: 440134

Other references

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