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Isolation and biological properties of virulent subpopulations from lentogenic Newcastle disease virus strains

, : Isolation and biological properties of virulent subpopulations from lentogenic Newcastle disease virus strains. Comparative Immunology, Microbiology and Infectious Diseases 2(4): 485-496

Virulent subpopulations of LaSota and Hitchner strains of Newcastle disease virus (NDV) were repeatedly but not consistently isolated from the lentogenic parent strains by chloroform extraction of infected allantoic fluid. The subpopulations were more resistant to chloroform and to heat than the parent strains; glycoproteins, haemagglutinin and F factor were also more stable to heat. Both subpopulations had NDV morphology (electron microscopy) and had the same elution kinetics from fowl red blood cells as the parent strains. Pathogenicity index in chicks and mean death time in chick embryos classified the subpopulations as virulent NDV.


PMID: 548205

DOI: 10.1016/0147-9571(79)90090-0

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