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Isolation and partial biochemical characterization of the brush border plasma membrane from the cestode, Hymenolepis diminuta

, : Isolation and partial biochemical characterization of the brush border plasma membrane from the cestode, Hymenolepis diminuta. Journal of Parasitology 65(5): 715-731

The tegument was removed from Hymenolepis diminuta using 0.2% Triton X-100 and brush border fragments were collected separately from cytoplasmic vesicles by differential centrifugation. The brush border plasma membrane was further enriched by disruption in high osmolarity Tris-HCl buffer. The distribution of plasma membrane was quantified by comparing the specific activity of 3H-concanavalin A in all fractions after incubation of intact organisms with the radioactive lectin.


PMID: 229204

DOI: 10.2307/3280351

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