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Properties of black gram mottle virus


, : Properties of black gram mottle virus. Phytopathology 69(4): 346-348

Blackgram mottle virus was purified from infected [Phaseolus vulgaris] 'Black Valentine' bean plants by a chloroform-butanol procedure. Purified preparations contained polyhedral particles 28 nm in diameter and exhibited a single immunoelectrophoretic component that migrated toward the anode at pH 8.8. Analytical ultracentrifugation showed a single component with a sedimentation coefficient of 122S and a density of 1.364 gm/cm3. Polyacrylamide gel electrophoresis demonstrated 1 nucleic acid component and 1 coat protein subunit with estimated MW of 1.4 .times. 106 and 38,200 daltons, respectively. Phenol-extracted nucleic acid was infectious on Pinto bean and sensitive to RNase and had a nucleotide ratio of G = 24.9%; A = 25.9%; C = 25.3%; U = 24.0%. Blackgram mottle virus was transmitted by the bean leaf beetle, Cerotoma trifurcata, and the Mexican bean beetle, Epilachna varivestis. Reciprocal serologic tests failed to show relationships between blackgram mottle virus and members of other beetle-transmitted legume virus groups.

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