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Survival of honey bee spermatozoa in liquid nitrogen

, : Survival of honey bee spermatozoa in liquid nitrogen. Annals of the Entomological Society of America 70(2): 257-258

Worker progeny was produced by queens inseminated with semen that had been stored in liquid nitrogen for 48 h. The cryoprotectant was DMSO (dimethyl sulphoxide). Without DMSO, the spermatozoa did not survive. Queens inseminated with a semen mixture (60% semen, 30% saline, 10% DMSO) that had been stored in liquid nitrogen retained 41% as many spermatozoa in their spermathecae as control queens.


DOI: 10.1093/aesa/70.2.257

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