A highly basic cyanogen bromide peptide from sowbane mosaic virus protein
Tremaine J.H.; Ronald W.P.; Kelly E.M., 1981: A highly basic cyanogen bromide peptide from sowbane mosaic virus protein. Virology 114(1): 282-285
The protein of sowbane mosaic virus (SoMV) was cleaved with CNBr and a highly basic peptide, sCB-1, was isolated by Sephadex and ion-exchange chromatography. The amino acid composition and sodium dodecyl sulfate-polyacrylamide gel electrophoresis indicated that sCB-1 had 49 amino acid residues. Eighteen residues are basic, 15 are lysine and 3 are arginine. sCB-1 move faster in polyacrylamide gel electrophoresis at pH 2.2 than a similar sized CNBr peptide from the bean strain of southern bean mosaic virus with 12 basic residues. The highly basic nature of sCB-1 suggests that this portion of the SoMV protein sequence is involved in protein-RNA binding in the virus particles.PMID: 18635096
DOI: 10.1016/0042-6822(81)90278-6
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