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A medium for the culture of Balantidium

, : A medium for the culture of Balantidium. Veterinariya, Moscow, USSR (7): 38

The preparation of a medium, ES-16, for the culture of Balantidium from pig is described: 7.0 g of sodium chloride, 1.0 g disubstituted sodium phosphate and 0.4 g monocalcium phosphate are dissolved in 1000 ml distilled water. The solution is sterilized by boiling for 15 min., filtered, topped-up to initial volume and the pH is brought to 7.3 to 7.6. Test tubes containing 8 ml of the medium are autoclaved and rice starch added. The medium has a shelf life of 45 days at 4 deg C.


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