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A method for determining tissue sulfate

, : A method for determining tissue sulfate. Analytical Biochemistry 118(2): 259-261

A method for the determination of the inorganic sulfate present in rat liver homogenates was developed. To determine sulfate, a protein-free extract is required. The classical protein precipitation methods of preparing protein-free extracts gave 2.5-40% recovery of added 35SO42-. Separation of the protein by ultrafiltration gave only 29% recovery when 0.15 M KCl was the homogenizing medium. A homogenization medium containing 0.154 M NH4OH and 20 g EDTA/l gave 102 .+-. 11% recovery of added 35SO42- when the protein was separated by ultrafiltration.


PMID: 6802023

DOI: 10.1016/0003-2697(81)90188-3

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