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A method for isolating continuous cell lines from Toxorhynchites amboinensis (Diptera: Culicidae)

, : A method for isolating continuous cell lines from Toxorhynchites amboinensis (Diptera: Culicidae). Journal of Medical Entomology, 182: 140-144

Minced larval tissue fragments and mechanically damaged but living whole larvae of Toxorhynchites amboinensis (Doleschall) were compared for initiating continuous cell lines. No cell line was obtained from completely minced larval tissue fragments, although patches of cells that adhered to the glass walls of the test-tube often replicated for 3-8 weeks. Over 20 continuous cell lines were isolated however from mechanically damaged but living larvae.


DOI: 10.1093/jmedent/18.2.140

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