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A method for the determination of available fluoride from marine products using young chicks

, : A method for the determination of available fluoride from marine products using young chicks. Journal of the Science of Food & Agriculture 32(5): 467-474

A method is described for the determination of available fluoride in biological materials. Groups of 1 day old male White Leghorn chicks were fed a low fluoride diet for 14 days (2.3 mg F- kg-1) to which graded levels of fluoride were added as sodium fluoride or in the form of fluoride-containing foods. The drinking water did not contain any detectable amount of fluoride. Water and feeds were provided ad lib. The response was measured as the concentration of fluoride in the 3 bones of the 3rd toe from both feet on a fat-free dry matter basis. A linear accumulation was observed for the addition of up to 500 mg F- kg-1 to the diet. An experimental dose range between 0-8 mg kg-1 was chosen. The availability of fluoride was measured as the slope ratio between highly correlated regression lines for test material and a standard. The lines had a common intersection on the .gamma.-axis. Relative to sodium fluoride, the biological availability of fluoride from different types of fish protein concentrate showed values of 110-151%. The corresponding values for krill (Meganyctiphanes norvegica) and a fluoridated toothpaste containing sodium monofluorophosphate were 112 and 145%, respectively.


PMID: 7253586

DOI: 10.1002/jsfa.2740320507

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