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A method for the iodination of insulin and its binding to dissociated mouse mammary cells


, : A method for the iodination of insulin and its binding to dissociated mouse mammary cells. Endocrinologia Japonica 28(1): 29-35

The iodination of insulin was accomplished by a modification of the lactoperoxidase method. The use of a low concentration of hydrogen peroxide (1.5 ng/.mu.l) followed by Sephadex gel filtration and purification on a cellulose column yielded iodoinsulin with an activity equal to that of native insulin in stimulation of glucose oxidation in rat epididymal fat cells and with high specific binding to collagenase-dissociated mouse mammary cells from pregnant and lactating mice. Other hormones tested did not displace the binding. Analysis of displacement curves and Scatchard plots suggests that both the affinity and the number of sites for insulin binding differ between pregnant and lactating mammary cells.

US$19.90

PMID: 7018891

DOI: 10.1507/endocrj1954.28.29


Other references

Ortiz J.M.; Berkeley R.C.W.; Brewer S.J., 1973: Production of exo beta n acetyl glucosaminidase by bacillus subtilis strain b. Journal of General Microbiology 77(2): 331-337

Kim, K.Han.; Kim, M.Chan.; Jung, G.Joong.; Kim, H.Ho., 2012: Long-term outcomes and feasibility with laparoscopy-assisted gastrectomy for gastric cancer. Recently, laparoscopy-assisted gastrectomy (LAG) has been widely accepted modality for early gastric cancer in Korea. The indication of LAG may be extended in an experienced institution. In our institution, the first case of laparoscopy-assisted g...

Lee, J.C.W.; Lennar Jones, J.E., 1998: Age difference at diagnosis between parents and children with inflammatory bowel disease Genetic anticipation of a cohort effect?. Gastroenterology 114(4 PART 2): A1019, April 15

Fauroux, B.; Baculard, A.; Boulé, M.; Tournier, G., 1995: Noninvasive ventilation using nasal mask in mucoviscidosis. Revue des Maladies Respiratoires 12(5): 509-511

Westra, A.; Dewey, W.C., 1971: Variation in sensitivity to heat shock during the cell-cycle of Chinese hamster cells in vitro. International Journal of Radiation Biology and Related Studies in Physics, Chemistry, and Medicine 19(5): 467-477

Quesada, A.; Allen, A.; Reeves, J., 1997: Financial characteristics of refrigerated food products trucking firms in the U.S. Journal of food distribution research 28(1): 74-81

Saylan, T.; Marks, R.; Jones, E.W., 1971: Fibrous papule of the nose. British Journal of Dermatology 85(2): 111-118

Loughry W.J., 1987: The dynamics of snake harassment by black tailed prairie dogs. Differential participation by particular classes of individuals is likely to influence how we assess the functional significance of harassing. I examined encounters between black-tailed prairie dogs (Cynomys ludovicianus) and snakes in both natura...

Seidel, K., 1954: Erroneous diagnosis of rheumatism and its differential-diagnostic evaluation. Munchener Medizinische Wochenschrift 96(36): 1015-8; Concl

Soares de Carvalho Gaspar, 1954: Sur les depots a galets calcaires du bassin du Mondego et les sables de la Gandara (Portugal). A granulometric and morphoscopic study of the limestone pebble deposits of the Mondego basin, Portugal. It is concluded that they were formed under the cold periglacial climatic conditions under which eolian sands of the Gandara area accumulated.