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A method of exclusion chromatography to obtain unitar kappa -casein

, : A method of exclusion chromatography to obtain unitar kappa -casein. Egyptian Journal of Dairy Science 9(2): 129-135

A kappa -casein rich fraction is obtained from acid casein dissolved in urea by addition of sulphuric acid and centrifugation, followed by addition of ammonium sulphate to the supernatant. 150 mg of this fraction is dissolved in 10 ml 10 mM Tris/6.6 mM citrate/6 M urea buffer (pH 8.6) and filtered through a column of Sephacryl S-200 superfine equilibrated with the same buffer.


Other references

Mclean, F.C.; Hastings, A.B., 1934: The state of calcium in the fluids of the body. Using the frog heart method it has been shown that in protein free fluids (cerebrospinal fluid) of human origin nearly all the Ca present is in the ionized form Ca combines with protein in solutions of purified serum proteins and the combination r...

Zhu, J-Ning.; Zhang, Y-Ping.; Song, Y-Ning.; Wang, J-Jun., 2004: Cerebellar interpositus nuclear and gastric vagal afferent inputs reach and converge onto glycemia-sensitive neurons of the ventromedial hypothalamic nucleus in rats. The glycemia-sensitive neurons of the ventromedial hypothalamic nucleus (VMN) have traditionally been implicated in feeding regulation. Some studies reported that the neuronal activity of the VMN could be modulated by inputs from the gastric vagal...

Doyle C.M.; Arp D.J., 1987: Regulation of hydrogen oxidation activity and hydrogenase protein levels by hydrogen oxygen and carbon substrates in alcaligenes latus. Regulation of H2 oxidation activity and hydrogenase protein levels in the free-living hydrogen bacterium Alcaligenes latus was investigated. Hydrogenase activity was induced when heterotrophically grown cells were transferred to chemolithoautotrop...

Dill, H.R., 1926: Is The Automobile Exterminating The Woodpecker?. Ten Nicotiana species were assayed for the proportion of DNA that is complementary to ribosomal RNA. This proportion varies from 0.27 to 0.9 percent, with tetraploid species having lower values than the diploid species. The tetraploid species have...

Abinawanto, K.S.imada; Saito, N.; Sugishima, T.; Shiokawa, E., 1997: Effects of aromatase inhibitor on sex differentiation and levels of P450C17 and P450arom mRNA of gonads in broiler embryos and broiler chickens. The objective of this study was to determine effects of non-steroidal aromatase inhibitor (Al, Fadrozole, Ciba-Geigy) on gonadal differentiation in broiler embryos and broiler chickens. At day 5 of incubation fertilized eggs (of hens) were injecte...

Mei, N.; Jeanningros, R.; Boyer, A., 1980: Role of vagal intestinal glucoreceptors in insulin regulation. This paper summarizes recent data on the existence of insulin nervous regulation by duodenal glucoreceptors which are connected to non-medullated fibres included in the vagal nerves. These glucoreceptors respond to intestinal perfusion of glucose...

Peacock, H.K.; Brandon, J.R., 1964: Experiences With Bilateral-Lateral Fusion Of The Lumbar Spine. Southern Medical Journal 57: 1417-1420

Henze, O., 1979: Das Ergebnis 40 jahriger gezielter Singvogelansiedlung zur Niederhaltung des Eichenwicklers. Falke, 261: 13-20

Smith F.R.; Ackers G.K., 1983: Probing the pathway of regulatory energy transduction in human hemo globin. Biophysical Journal 41(2 PART 2): 415A

Shaposhnikova, N.F.; Poliakov, N.F., 1972: Nucleic acids in the ciliary epithelium of the rabbit normally and during stimulation of the hypothalamus. Vestnik Oftalmologii 5: 86-88