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A method of producing interspecific hybrid forms of potato

, : A method of producing interspecific hybrid forms of potato. USSR Patent: (731934)

To accelerate the production of interspecific hybrids, isolated protoplasts are treated before fusion with a 40-50% solution of polyethylene glycol at room temperature for 15-25 min. They are then soaked once or twice in a 0.16-0.17 M solution of Ca(NO3)2 and cultured after fusion on modified Murashige-Skoog medium until callus formation.


Other references

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Yajima H., 1984: Total synthesis of bovine pancreas rnase a. Yakugaku Zasshi 104(1): 1-10

Mubarak, A.; Klages, M.G.; Olsen, R.A., 1978: An improved moistening technique for aggregate stability measurement. An ultrasonic nebulization technique has been used for uniform wetting of soil by introduction of an aerosol. Values obtained for aggregate stability of air-dried soils were greater when this technique was used than with conventional wetting techn...

Kinno, R.; Yamazaki, T.; Yamamoto, M.; Takahashi, Y.; Fukui, T.; Kinugasa, E., 2013: Cerebellar symptoms in a case of acute limbic encephalitis associated with autoantibodies to glutamate receptors δ2 and ɛ2. Clinical Neurology and Neurosurgery 115(4): 481-483

Davis, D.R.; Seth, P.P., 2011: Therapeutic targeting of HCV internal ribosomal entry site RNA. HCV infection is a significant human disease, leading to liver cirrhosis and cancer, and killing >10,000 people in the US annually. Translation of the viral RNA genome is initiated by ribosomal binding to a highly structured RNA element, the in...

Tsukada, M., 1980: Thermal characteristic of silk. Nihon sanshigaku zasshi = The Journal of sericultural science of Japan 49(2): 167-168

Pachkov, M.; Erb, I.; Molina, N.; van Nimwegen, E., 2006: SwissRegulon: a database of genome-wide annotations of regulatory sites. SwissRegulon ( is a database containing genome-wide annotations of regulatory sites in the intergenic regions of genomes. The regulatory site annotations are produced using a number of recently developed algorith...

Zahari, M.W.; Alimon, A.R., 2004: Use of palm kernel cake and oil palm by-products in compound feed. This article discusses palm kernel cake (PKC) utilization in beef cattle, buffaloes, dairy cattle, sheep, goats, poultry, swine and aquaculture in Malaysia. It also discusses recommended levels of PKC in livestock rations, current research on the...

Huang, S-Hai.; Feng, X-Liang.; Liu, W., 2009: Study on the inhibitory effect of Reduqing Oral Liquid on respiratory syncytial virus in vitro. To explore the antiviral effect of Reduqing Oral Liquid on respiratory syncytial virus (RSV). The anti-RSV effect of Reduqing Oral Liquid in Hep-2 cell culture was investigated by the cytopathic effect (CPE) inhibition assay and MTT assay, with ri...

Kleaveland, R.N., 1961: Internal-carotid-artery embolectomy. New England Journal of Medicine 264: 759-761