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A study of phosphoenolpyruvate carboxykinase from Moniliformis dubius (Acanthocephala)

, : A study of phosphoenolpyruvate carboxykinase from Moniliformis dubius (Acanthocephala). Molecular and Biochemical Parasitology, 23-4: 151-166

Procedures have been developed for the extraction and subsequent purification of the enzyme phosphoenolpyruvate carboxykinase (GTP) (PEPCK) from Moniliformis dubius (Acanthocephala), a parasite of the rat small intestine. This is believed to be the first purification of PEPCK from an invertebrate animal. The enzyme, when purified to homogeneity as indicated by electrophoretic criteria, has a molecular weight of 73 700. Kinetic studies indicated that the enzyme had a pH optimum of 5.5 and required Mn2+ as the divalent cation. The apparent Km values determined for the substrates of the carboxylation reaction were low compared with the published values for purified PEPCK from vertebrate sources. Several competitive inhibitors were found and their Ki values determined. The possible regulation of PEPCK activity in M. dubius is discussed with reference to the observed kinetic parameters.


PMID: 7219443

DOI: 10.1016/0166-6851(81)90096-7

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