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Evaluation of different culture media for isolation of Leishmania donovani


, : Evaluation of different culture media for isolation of Leishmania donovani. Journal of Communicable Diseases 12(4): 188-191

Bone marrow aspirates from children with suspected L. donovani infection were inoculated into 3 different media. Parasites were detected in bone marrow smears from 19 of the patients. The highest rate of isolation (17 of 20) was obtained in semi-solid agar with 10 or 15% defibrinated rabbit blood at pH 7.4, and promastigotes were detected earlier in this medium.

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