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Large-scale fractionation of proteins by isoelectric focusing in a multi-compartment electrolysis apparatus

, : Large-scale fractionation of proteins by isoelectric focusing in a multi-compartment electrolysis apparatus. Electrophoresis 1(1): 3-14

A preparative electrolysis apparatus for isoelectric focusing of large amounts of proteins is described. It contains 46 separation compartments, and its total vol. is approx. 7.6 l. The compartments are closed, and internal cooling and stirring are effected by slow rotation of the whole apparatus in a tank filled with cold water. The apparatus can be run with an electric load of up to 5 kW, and isoelectric focusing takes 2-3 days.


DOI: 10.1002/elps.1150010103

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