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Long term cultivation of a simian malaria parasite (Plasmodium knowlesi) in a semi-automated apparatus

, : Long term cultivation of a simian malaria parasite (Plasmodium knowlesi) in a semi-automated apparatus. Transactions of the Royal Society of Tropical Medicine and Hygiene 74(6): 789-792

An apparatus is described and illustrated for the continuous semi-automated cultivation of Plasmodium knowlesi. The change of medium was automated and involved six operations. Parasites were maintained for 12 weeks, at which time the experiment ended. During this period, parasite density, morphology and serological specificity were monitored. Parasites retained synchromy and normal morphology only for the first cycle in culture. The maximum degree of morphological abnormality (90%) was reached at three weeks. Lysis of infected erythrocytes seemed to occur before full maturation of the parasites. Infected cells cultured for 31 days produced a normal, fulminating infection in a rhesus monkey.


PMID: 7210132

DOI: 10.1016/0035-9203(80)90202-3

Other references

Potsic, W.P., 1987: Comparison of polysomnography and sonography for assessing regularity of respiration during sleep in adenotonsillar hypertrophy. Increasing awareness of the role of adenotonsillar hypertrophy in the etiology of chronic airway obstruction and disturbed respiration during sleep has created interest in the diagnostic methods available to assess the effects of obstruction. This...

Fujii T.; Fujii E., 1975: Effect of thyroidectomy on the tritiated estradiol uptake and disappearance in the uterus pituitary ovary and adrenal in adult rats. Japanese Journal of Pharmacology 25(SUPPL): 164P-165P

Welt, T.; Engelmann, M.; Renner, U.; Erhardt, A.; Müller, M.B.; Landgraf, R.; Holsboer, F.; Keck, M.E., 2006: Temazepam triggers the release of vasopressin into the rat hypothalamic paraventricular nucleus: novel insight into benzodiazepine action on hypothalamic-pituitary-adrenocortical system activity during stress. We investigated the influence of a representative classical benzodiazepine on the regulation of the hypothalamic-pituitary-adrenocortical (HPA) axis activity both under basal conditions and stress. Adult male Wistar rats were intravenously admini...

J.R.Bowman, 1992: The 1988 Tennant Creek, Northern Territory, earthquakes; a synthesis. Australian Journal of Earth Sciences 39(5): 651-669

Takahashi, S.; Nakajima, M.; Ikeda, Y.; Kondo, S.; Hamada, M., 1971: A new antibiotic, cyclamidomycin. Journal of Antibiotics 24(12): 902-903

Alt, GL., 1981: Reproductive biology of black bears of northeastern Pennsylvania. Transactions of the Northeast Fish and Wildlife Conference, 38: 88-89

Poulton, E.C.; Hunt, J.C.; Mumford, J.C.; Poulton, J., 1975: The mechanical disturbance produced by steady and gusty winds of moderate strength: skilled performance and semantic assessments. Ergonomics 18(6): 651-673

Fung, A.; Rayter, Z.; Fisher, C.; King, D.M.; Trott, P., 1990: Preoperative cytology and mammography in patients with single-duct nipple discharge treated by surgery. Microdochectomy has been the routine management for single-duct nipple discharge. Our review of 176 consecutive patients surgically treated between 1975 and 1988 revealed eight patients with in situ carcinomas and seven with infiltrative carcinoma...

Decara, J.M.; Dubrey, S.; Falk, R.H., 2000: Going going gone The relationship between atrial fibrillation duration and atrial stunning. Journal of the American College of Cardiology 35(2 suppl A): 187A-188A, Feb

Divakov V.I.; Chistyakov V.B., 1987: Differential solidification of clay and the barich heterogeneity of geological section of eastern Caucasus. Trudy Vsesoyuznogo Neftyanogo Nauchno-Issledovatel'skogo Geologorazvedochnogo Instituta 467: 84-92