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An enzyme immunoassay of estrone in swine serum

, : An enzyme immunoassay of estrone in swine serum. Steroids 39(6): 657-666

An enzyme immunoassay for estrone in swine serum was established. For this, .beta.-galactosidase from Escherichia coli was conjugated through estrone 17(O-carboxymethyl)oxime using a mixed anhydride reaction. The percentage of immunoreactive estrone-17(O-carboxymethyl)oxime-.beta.-galactosidase conjugate was estimated at .apprx. 70%. The recovery rate of estrone (25-500 pg) added to 0.05 ml of swine serum averaged 91.4%. The sensitivity of the present enzyme immunoassay was 5 pg/tube. The coefficients of variation (CV) were 5.9-8.2% (within assays) and 4.1-5.9% (between assays), respectively. Estrone values determined by the present enzyme immunoassay were highly correlated with those determined by radioimmunoassay (r = 0.99, P < 0.005). This method of enzyme immunoassay was determined to be suitable for the routine assay of serum estrone [which serves as an indicator of the fetal placental function.].


PMID: 6760470

DOI: 10.1016/0039-128x(82)90136-2

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