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Endopeptidase activity in jackbeans and its effect on Concanavalin A

, : Endopeptidase activity in jackbeans and its effect on Concanavalin A. Planta 157(6): 531-535

Endopeptidase activity in mature jackbeans (Canavalia ensiformis) was characterized. One major activity was present, that of a neutral metallo-endopeptidase. Using specific inhibitors it was shown that the concanavalin A-associated fragments were not formed by proteolytic degradation of the intact subunit on hydration of the tissue. The intact subunit of the lectin was also resistant to the degradation by the endogenous endopeptidase activity during a prolonged incubation in vitro. These results suggest that the lectin fragments form during seed maturation and no further limited proteolysis of concanavalin A occurs during the early stages of germination.


PMID: 24264418

DOI: 10.1007/BF00396884

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