geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Histochemical studies of glycosidase activity in juveniles and adults of the lung fluke Paragonimus


, : Histochemical studies of glycosidase activity in juveniles and adults of the lung fluke Paragonimus. Parasitology 86: 119-126

Glycosidase activities in the adults and juveniles of the lung fluke Paragonimus ohirai and P. westermani adults were demonstrated histochemically. For comparative studies, histochemical examination was also made on the adults of the liver fluke Fasciola hepatica. The enzymes examined were N-acetyl-beta-glucosaminidase (EC 3.2.1.30), beta-glucuronidase (EC 3.2.1.31), beta-galactosidase (EC 3.2.1.23), alpha-glucosidase (EC 3.2.1.20) and beta-glucosidase (EC 3.2.1.21). The distribution of beta-glucosaminidase was similar in juveniles and adults. Strong reaction sites for the enzyme were the caecal brush border, tegument, subtegumental cells and tests. In contrast, no staining reaction occurred in the caeca of F. hepatica, although the tegument and parenchymal cells were weakly stained. beta-glucuronidase activity was associated only with the luminal surface of the caeca in the juveniles. However, luminal contents also appeared stained and this might suggest that the activity in the caeca is not endogenous. beta-galactosidase was localized in the caeca, sub-tegmental cells and tegument in both juveniles and adults. No reaction occurred for the other two enzymes, alpha- and beta-glucosidase.

US$19.90

PMID: 6220258

DOI: 10.1017/s003118200005722x


Other references

Csendes, A.; Smok, G.; Burdiles, P.; Quesada, F.; Huertas, C.; Rojas, J.; Korn, O., 2000: Prevalence of Barrett's esophagus by endoscopy and histologic studies: a prospective evaluation of 306 control subjects and 376 patients with symptoms of gastroesophageal reflux. The classic endoscopic diagnosis of a Barrett's esophagus (BE) is based on the finding of > or =3 cm, of distal esophagus covered by specialized columnar epithelium. However, currently, it is based on the finding of intestinal metaplasia (...

Vysotskii, V.; Martinchik, A.; Bondarev, G.; Mamaeva, E.; Feoktistova, A.; Chernov, N.; Peskova, E.; Zhminchenko, V., 1990: Investigation of the activity of enzymes participating in metabolism of xenobiotics for evaluating the quqlity of protein-containing products of microbial synthesis. The activity of enzymes participating in the metabolism of xenobiotics in the liver of rats fed protein products obtained by the microbial synthesis method was investigated to evaluate their quality and harmlessness. It is shown that the biomass o...

Anonymous, 1970: Is rheumatic fever necessary?. JAMA 214(2): 361-362

Rutland, P.S.; Khal, M., 1985: Description of mungbean cultivar 'King'. Mungbean cv. King, derived from a selection of line CPI 64204, was released through the Grain Legume Registration Scheme in 1982. King is similar in morphological detail to the accepted norm for mungbean (Vigna radiata), and compares favourably wi...

Dame, G.G.oeckner, G.B.ck, C., 2002: Knock-out of a putative transporter results in altered blue-light signalling in Chlamydomonas. Nitrogen starvation and blue light are the two environmental cues that control sexual differentiation in Chlamydomonas reinhardtii. Insertional mutagenesis was applied to generate mutants that still require nitrogen starvation as the initiating si...

Johnsen, T.; Friis, M.L., 1980: Paramyotonia congenita (von Eulenburg) in Denmark. Paramyotonia congenita (von Eulenburg) (PMC) is described in a Danish family in seven generations and 39 persons. PMC has not previously been described in Denmark and an epidemiological description of the family is given. The diagnostic difficulti...

Supekar, P.; Rawat, S.; Chaudhary, D., 1969: Improvement of Chhattisgarh cattle--a timely need. Gosamvardhana 17 (7 8) 3-5

Kishimoto, T., 1968: Experimental studies on the influences of diathermy coagulation of retina on ERG. 2. Nonperforative coagulation. Nippon Ganka Gakkai Zasshi 72(2): 123-129

Vaghi M.A.; Bruzzone M.G.; Visciani A.; Passerini A., 1985: Intracranial tumors arising from the floor of the middle fossa. Tumors involving or arising from the floor of the middle fossa in cases seen at the Neurological Institute C. Besta were reviewed, and the radiological findings of plain films, angiographic and CT studies are described. Meningiomas, metastases, tr...

Murphy, M.E.; Kolvenbach, R.; Aleksis, M.; Hansen, R.; Sies, H., 1992: Antioxidant depletion in aortic crossclamping ischemia: increase of the plasma alpha-tocopheryl quinone/alpha-tocopherol ratio. To assess whether oxidative stress contributes to the ischemia/reperfusion injury of aortic surgery, the contents of alpha-tocopherol, alpha-tocopheryl quinone, ascorbate, lipid-derived malondialdehyde, protein thiols, cholesterol, and lactate wer...