geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Factors influencing lipolysis by skim milk cultures of some psychrotrophic microorganisms


, : Factors influencing lipolysis by skim milk cultures of some psychrotrophic microorganisms. Australian Journal of Dairy Technology 38(3): 97-103

Pseudomonas fluorescens, Moraxella spp., Acinetobacter spp. and P. aeruginosa isolates from raw milk were cultured in sterile skim milk and the extracellular lipases present in crude form in the cell-free supernatants were examined. They hydrolyzed tributyrin, emulsified milk fat and cream at comparable rates under optimal conditions of pH 7.5-9.0 and 40.degree.-50.degree. C. Bile salts stimulated their lipolysis of milk fat, but had a variable influence on the lipolysis of cream. The effects of several other activators were also examined. NaCl to 2.18M reduced lipolysis by, on average, < 1/3. Certain metal ions at 2-10mM (i.e., Fe3+, Zn2+) were inhibitory, particularly to Acinetobacter lipases. EDTA caused irreversible loss of activity in the Acinetobacter preparations, whereas the activity of those of P. fluorescens and Moraxella was restored by Ca2+. The activities in P. fluorescens and Moraxella cultures were only partially (.ltoreq. .apprx. 60%) inactivated by heating at 100.degree. C for 30s. The lipase activity in a skim milk culture of the yeast Cryptococcus albidus had properties considerably different from those of the bacterial lipases.

US$29.90


Other references

Bonfils, P.; Trotoux, J., 1989: Mucormycosis of the face associated with aspergillosis in the diabetic patient. Diagnostic and therapeutic considerations. Facial mucormycosis is a rare fungal infection which spreads rapidly and has a severe prognosis. It mainly affects insulin-dependent diabetics and immunodeficient subjects. We report about a case of mucormycosis of the facial structures in a diabe...

Morris, R.F., 1961: Life-history and habits in Newfoundland of Swammerdamia lutarea (Haw.) (Lepidoptera: Tponomeutidae), a pest new to North America. The European Hyponomeutid, Swammerdamia lutarea (Haw.), all stages of which are briefly described, was recorded for the first time in North America in June 1954, when the larvae severely infested ornamental hawthorns (Grataegus oxyacantha and C. p...

Wolpert L.; Gingell D., 1969: The cell membrane and contact control. Wolstenholme, G E W And Julie Knight (Edited By) A Ciba Foundation Symposium Homeostatic Regulators Viii + 327p Illus J And A Churchill Ltd : London, England 241-259

Everson R.B.; Gad E.M.wla N.M.; Attia M.A.M.; Chevlen E.M.; Thorgeirsson S.S.; Alexander L.A.; Flack P.M.; Staiano N.; Ziegler J.L., 1983: Analysis of human urine for mutagens associated with carcinoma of the bilharzial bladder by the ames salmonella plate assay interpretation employing quantitation of viable lawn bacteria. The Ames Salmonella plate assay was employed to test urine samples from bladder cancer patients and controls living in Egypt for the presence of chemical mutagens. Urine from 5 groups of Egyptian adults were tested, including individuals with neit...

Liska, J.; Slovácek, R.; Varvarovská, J.; Zavázal, V.; Mracková, E., 1985: Immunologic reactivity of children with idiopathic nephrotic syndrome. Ceskoslovenska Pediatrie 40(8): 453-457

Akoumianakis, I.; Antoniades, C., 2016: Is stress response a new link between adipose tissue and atherogenesis? The role of HSPs/HSF1. Cardiovascular Research 111(1): 10-12

Floroff, C.K.; Hassig, T.B.; Cochran, J.B.; Mazur, J.E., 2016: High-Dose Sedation and Analgesia During Extracorporeal Membrane Oxygenation: A Focus on the Adjunctive Use of Ketamine. Use of ketamine in patients requiring extracorporeal membrane oxygenation (ECMO) has rarely been reported, and the optimal dosing strategy remains unclear. A patient admitted with hypoxic respiratory failure required ECMO in addition to continuous...

Suchman, A.L.; Condemi, J.J.; Leddy, J.P., 1983: Seizure after pulse therapy with methyl prednisolone. Arthritis and Rheumatism 26(1): 117-117

Marx, R., 1965: Some experiences in the substituion therapy of the factors VH and X in prothrombin complex deficiencies. Proc Congr Int Soc Blood Transfus 10(5): 1348-1349

Benthe, H.F.; Knop, J.; Schmoldt, A., 1972: Absorption and distribution of poly chlorinated bi phenyls after inhalatory application. Archives of Toxicology 29(2): 85-95