geoscience.net logo
+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Identification of a connecting filament protein in insect fibrillar flight muscle


, : Identification of a connecting filament protein in insect fibrillar flight muscle. Journal of Molecular Biology 153(3): 661-680

A major component on sodium dodecyl sulfate-containing gels of solubilized isolated Z-discs, purified from honeybee flight muscle, migrates with an apparent MW of 360,000. Antibodies to this high MW polypeptide were prepared by injecting rabbits with homogenized gel slices containing the protein band. With indirect immunofluorescence microscopy these antibodies are localized to a region extending from the edge of the Z-band to the A-band in shortened or stretched sarcomeres. Glycerinated flight muscle treated with antiserum and prepared for EM shows enhanced density from the ends of the thick filaments to the I-Z junction regardless of sarcomere length. Antiserum is directed toward a structural protein of connecting filaments, which link thick filaments to the Z-band in insect fibrillar muscle, rather than to a thin filament component. In Ouchterlony double-diffusion experiments a single precipitin band is formed when antiserum is diffused against solubilized Z-discs; no reaction occurs between antiserum and proteins from native thin filaments prepared from honeybee light muscle. Antibody stains the I-band in flight muscle fibrils from which thin filaments are removed. Honeybee leg muscle myofibrils, in which connecting filaments are not observed, are not labeled with antibody. Since antibody binds to the short projections which extend from the flat surfaces of isolated Z-discs, these projections are assumed to be remnants of connecting filaments and the source of the 360,000 MW protein. The amino acid composition of this high MW material, purified by Sepharose chromatography, is presented. The protein was named projectin.

US$19.90

PMID: 6802982

DOI: 10.1016/0022-2836(81)90412-5


Other references

Brundelet, P.J., 1973: Acid-fast staining of hooklets of Taenia echinococcus. Hydatid hooklets were revealed when 2 surgical specimens, one an intrahepatic cystic mass and the other a paravertebral abscess, were stained by the Ziehl-Neelsen technique. The hooklets were acid-fast and stained red in contrast to the pale blue...

Dony J.; Siemerink M.; Van Leusden H.A., 1971: Influence of cortico steroids on human placental steroidogenesis in vitro. Acta Endocrinologica Supplementum 155: 131

Farley G.R.; Barlow S.M.; Netsell R.; Chmelka J.V., 1992: Vocalizations in the cat behavioral methodology and spectrographic analysis. Attempts to understand the neural mechanisms underlying mammalian vocal behaviors, including speech, require study of the neural activity and anatomy of vocalization-controlling brain structures. Such studies necessiate the application of invasive...

Wu, H.; Reali, R.S.; Smith, D.A.; Trachtenberg, M.C.; Li, J., 2010: Highly selective CO2 capture by a flexible microporous metal-organic framework (MMOF) material. Chemistry 16(47): 13951-4

Sisco, M.; Mustoe, T.A., 2003: Animal models of ischemic wound healing. Toward an approximation of human chronic cutaneous ulcers in rabbit and rat. Methods in Molecular Medicine 78: 55-65

Ford, N.B.; Shuttlesworth, G.A., 1986: Effects of variation in food intake on locomotory performance of juvenile garter snakes. Copeia(4): 9-1001

Werner J.C.; D.M.ttos H.B., 1972: Studies on the fertilization of centrosema pubescens. Boletim de Industria Animal 29(2): 375-391

Narayana, L.; Rao, D., 1976: Contributions to the floral anatomy of Linaceae. 6. Journal of Japanese botany: 51 (3) 92-96

Padmanabhan, R.; Howard, T.H.; Howard, B.H., 1987: Specific growth inhibitory sequences in genomic DNA from quiescent human embryo fibroblasts. We used HeLa cells as recipients in a gene transfer assay to characterize DNA sequences that negatively regulate mammalian cell growth. In this assay, genomic DNA from quiescent human embryo fibroblasts was more inhibitory for HeLa replication tha...

Espinoza, E.O.; Scanlan, M.D.; McClure, P.J.; Baker, B.W., 2012: Forensic analysis of black coral (Order Antipatharia). Fourier-transform infrared spectroscopy (FTIR), discriminate analysis, X-ray fluorescence spectrometry (XRF), and stereoscopic microscopy were used to separate black coral forensic evidence items from similarly appearing items manufactured from pl...