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Nutritional factors affecting extracellular enzyme production by Pseudomonas fluorescens B52


, : Nutritional factors affecting extracellular enzyme production by Pseudomonas fluorescens B52. Milchwissenschaft 38(12): 705-707

Pseudomonas fluorescens B52, isolated from raw milk, was grown in a minimal salts medium (1% NH4Cl, 1% Na2HPO4, 0.2% KH2PO4, 0.05% CaCO3 and 0.05% MgSO4) supplemented with 3% spray-dried sweet whey, 1% casein or 3% whey + 1% casein. Incubation was at 7 degrees C for 14 days with agitation at 70 rev/min. No lag phase of growth in any of the 3 media was observed at this low temp. even though the inoculum had been grown at 25 degrees C for 16 h in nutrient broth.

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