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Somatic embryogenesis in stationary phase suspension cultures derived from hypocotyl protoplasts of Brassica napus L

, : Somatic embryogenesis in stationary phase suspension cultures derived from hypocotyl protoplasts of Brassica napus L. Plant Cell, Tissue and Organ Culture 12(2): 141-146

Hypocotyl protoplasts of winter swede rape line EGK10 were isolated and underwent several passages of culture on different media to encourage embryoid development and shoot formation. Aggregates derived from the stationary or late stationary phase of the growth cycle were the only suitable inoculum for embryoid formation. To obtain organized structures, the suspension had to be cultured for 20-40 days without any medium exchange.


DOI: 10.1007/bf00040076

Other references

Bludau Wolfgang; Feldmann Ludger, 1994: Geological, geomorphological and palynological research on the problem of dead ice south of Lake Starnberger. Eiszeitalter und Gegenwart 44: 114-128

Mishev, K., 1986: Geography and ecology.. Priroda (Sofia): 5: 3-8

Robertson, P., 1987: The release and survival of hand reared pheasants on an unkeepered estate in Ireland. Game Conservancy Annual Review, 82-86 No. 18

Oschilewski, M.; Schwab, E.; Kiesel, U.; Opitz, U.; Stünkel, K.; Kolb-Bachofen, V.; Kolb, H., 1986: Administration of silica or monoclonal antibody to Thy-1 prevents low-dose streptozotocin-induced diabetes in mice. Multiple injections of low doses of streptozotocin induce an experimental diabetes in mice. We have analyzed in two inbred strains whether the development of hyperglycaemia can be influenced by administration of macrophage-toxic silica particles o...

Chapman, BR.; Grantland, TL., 1981: Texas county records for bird specimens in the Corpus Christi State University Collection. Bulletin of the Texas Ornithological Society, 141-2: 26-27

Nienaber, A.; Huber, A.; Göttfert, M.; Hennecke, H.; Fischer, H.M., 2000: Three new NifA-regulated genes in the Bradyrhizobium japonicum symbiotic gene region discovered by competitive DNA-RNA hybridization. The so-called symbiotic region of the Bradyrhizobium japonicum chromosome (C. Kündig, H. Hennecke, and M. Göttfert, J. Bacteriol. 175:613-622, 1993) was screened for the presence of genes controlled by the nitrogen fixation regulatory protein Ni...

Haghipour A.; Marcelo M., 1968: Preliminary geology of the area northeast of Bernstein, Austria. Austr: . Bundesanst., Verh.. 3; Pages 97. 1968.

Tsurumi, H.; Anzai, H., 1940: Some clinical observations on a typhoid-like disease from a new strain, Salmonella onarimo. The author isolated a new strain of Salmonella from blood and stools of a dysentery patient. The somatic antigen was found to be of I, IX and XII, and its flagellar antigen consisted of a specific phase b, and of a nonspecific phase 1,2. The organ...

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Ornosa, Concepcion., 1996: A cautionary note on the artificial introduction of foreign subspecies of pollinating bees to the Iberian Peninsula Hymenoptera Apidae, Bombinae Una nota de atencion sobre la introduccion artificial de subespecies foraneas de abejorros polinizadores en la Peninsula Iberica Hymenoptera Apidae, Bombinae. Boletin de la Asociacion Espanola de Entomologia. Junio; 201-2: 259-260