+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Evaluation of new antiviral agents. I. In vitro perspectives

, : Evaluation of new antiviral agents. I. In vitro perspectives. Antiviral Research 11(5/6): 217-232

A variety of different methods for the evaluation of antiviral agents in cell culture systems are briefly reviewed. It has been repeatedly noted that many test conditions such as the cell culture system, virus strain, virus challenge dose, virus input multiplicity of infection, and time of harvesting, etc., can substantially affect or even alter the test results, thus making comparative studies and unambiguous evaluations very difficult. Attempts are made to discuss previous test methods together with our recent studies with the aim to simplify test procedures and assay methods. Suggestions are proposed for in vitro evaluation of new antiviral agents. It is hoped that this review will alarm investigators to the problems of assaying new antiviral agents. If the suggestions made in this review can be followed, the screening of the enormous number of promising antiviral compounds may be made more efficiently in the near future.


PMID: 2679375

DOI: 10.1016/0166-3542(89)90032-6

Other references

Burk, D.; Sadler, T.W.; Langman, J., 1979: Distribution of surface coat material on nasal folds of mouse embryos as demonstrated by concanavalin A binding. 3H-concanavalin A and the concanavalin A-horseradish peroxidase staining technique were used to study the distribution of surface coat material on the epithelium of the nasal folds and nasal groove of mouse embryos. In stages shortly before and du...

Schuurman, R.; Group, D.V.rology, 1996: Multicenter comparison of three commercial methods for plasma HIV-1 RNA quantification using spiked human plasma and clinical plasma samples. INFECTIOUS DISEASES SOCIETY OF AMERICA [Author], NATIONAL INSTITUTES OF HEALTH [Author], CENTERS FOR DISEASE CONTROL AND PREVENTION [Author] 3rd Conference on retroviruses and opportunistic infections : 153

Hill, R.D.; Craker, B.A., 1968: The role of lysine residues in the coagulation of casein. Coagulation of rennin-treated casein was inhibited by treatment of the casein with dimethylaminoaphthalene sulfonyl chloride. The effect was caused by substitution on lysine side chains of the k-casein fraction, and inhibition was complete when 2-...

Kader, F.I.mouli, M.N.colas, J.; Metche, M., 2001: Proposed mechanism for the degradation of pelargonidin 3-glucoside by caffeic acid o-quinone. Caffeic acid o-quinone (CQ) was prepared by oxidation of caffeic acid with o-chloranil in organic media. The reaction between the purified CQ and pelargonidin 3-glucoside (Pg 3-glc, non-o-diphenolic anthocyanin) was monitored by HPLC and spectroph...

Sorial, E., 1982: Analysis of EWUP farm management data for the Abyuha site. The characteristics of farms at the El Minya project site of the Egypt Water Use and Management Project (EWUP) are described. The farms for which records are kept are compared with the average of farms served by the Abyuha cooperative. Record keep...

Ang, C., 2016: Role of the fibroblast growth factor receptor axis in cholangiocarcinoma. Advanced cholangiocarcinoma (CCA) is a highly lethal disease with limited therapeutic options beyond cytotoxic chemotherapy. Molecular profiling of CCA has provided insights into the pathogenesis of this disease and identified potential therapeuti...

Scarano, E., 1958: Enzymatic synthesis of 5'-desoxyuridylic acid. Bollettino Della Societa Italiana di Biologia Sperimentale 34(14): 722-724

Davies, M.K.; Hollman, A., 2001: Cardiac risk factors. Heart 87(1): 5-5

Jennison, S.H.; Hossein Nia, M.; Brown, P.; Murday, A.J.; Holt, D.W.; Mckenna, W.J., 1992: Creatine kinase isoforms as an early marker of myocardial cell injury during acute allograft rejection. Circulation 86(4 SUPPL 1): I841

Weiss, N.S.; Silverman, D.T., 1977: Laterality and prognosis in ovarian cancer. Data from the End Results Group file at the National Cancer Institute indicate that in primary ovarian cancer neither the stage of disease at diagnosis nor patient survival is related to which of the two ovaries is first involved. Women with cance...