+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Transport of phenylalanine into vacuoles isolated from barley mesophyll protoplasts

, : Transport of phenylalanine into vacuoles isolated from barley mesophyll protoplasts. Planta 176(3): 378-382

The energy-dependent transport of phenylalanine into isolated vacuoles of barley (Hordeum vulgare L.) mesophyll protoplasts has been studied by silicone-layer floatation filtering. The uptake of this aromatic amino acid into the vacuolar compartment is markedly increased by MgATP, showing saturation kinetics; the Km values were 0.5 mM for MgATP and 1.2 mM for phenylalanine. Vmax for phenylalanine transport was estimated to 140 nmol phenylalanine .cntdot. (mg .cntdot. Chl)-1 .cntdot. h-1. The transport shows a distinct pH optimum at 7.3 and is markedly inhibited by 40 mM nitrate. Azide (1 mM) and vanadate (400 .mu.M) had no or little effect on rates of transport while p-fluorophenylalanine seemed to be an effective inhibitor, indicating a possible competition at an amino-acid carrier. Ionophores such as valinomycin, nigericin or gramicidin were strong inhibitors of phenylalanine transport, indicating that this process is coupled to both the transmembrane pH gradient (.DELTA.pH) and the transmembrane potential (.DELTA.psi.).


PMID: 24220866

DOI: 10.1007/BF00395418

Other references

Bruno, J.F.; Edmunds, P.J., 1998: Metabolic consequences of phenotypic plasticity in the coral Madracis mirabilis The effect of morphology and water flow on aggregate respiration. Phenotypic plasticity has been documented in a number of reef coral species for a variety of morphological traits, but its ecological importance is not well understood. In the branching coral Madracis mirabilis (Duchassaing and Michelotti) spacing...

Seelig, M.S., 1994: Magnesium, antioxidants and myocardial infarction. Journal of the American College of Nutrition 13(2): 116-117

Plichet, A., 1953: Migrations; new data. La Presse Medicale 61(56): 1147-1148

Gubanov, I.; Alekseev, I., 1975: New findings of rare and remarkable plants in the Moscow district. Biulleten' Otdel biologicheskii: 80 (3) 128-130

Sproul, John Timothy, 1994: Trends in Japan-USA seafood trade, with an emphasis on Alaska pollock surimi, and the effects on Japanese household consumption of surimi-based foods. Memoirs of the Faculty of Fisheries Hokkaido University 40(1-2): 1-123

Votava, V.; Bohut, V., 1967: Direct lymphadenography of the mediastinal lymph nodes. Les Bronches 17(5): 379-383

Gibbs C.L., 1976: Skeletal muscle energetics a review. Proceedings of the Australian Physiological & Pharmacological Society 7(2): 77-80

Holme, J.A.; Dybing, E., 1982: Induction of liver microsomal cytochrome P-450 and associated monooxygenases by octachlorostyrene in inbred strains of mice. Lack of correlation with the murine Ah locus. Single intraperitoneal injections of octachlorostyrene (OCS) and hexachlorobenzene in genetically polycyclic aromatic hydrocarbon "responsive' C57/BL/6 (B6) mice led to a time- and dose-dependent increase in the levels of liver microsoma...

Pongraczne,A.B.; Mezei,Z.; Gyori,Z.; Sipos,P., 2008: The impact of production site on the quality of wheat used for quality improvement in 2006-2007. Changes in the quality parameters (baking quality, wet gluten content, gluten expensiveness, gluten index, alveographical W value) of winter wheat varieties (Bitop, Mv Suba, Saturnus, Sixtus, Mv Mazurka, KG Szephalom) with improving quality charac...

Theesfeld, C.L.; Pourmand, D.; Davis, T.; Garza, R.M.; Hampton, R.Y., 2011: The sterol-sensing domain (SSD) directly mediates signal-regulated endoplasmic reticulum-associated degradation (ERAD) of 3-hydroxy-3-methylglutaryl (HMG)-CoA reductase isozyme Hmg2. The sterol-sensing domain (SSD) is a conserved motif in membrane proteins responsible for sterol regulation. Mammalian proteins SREBP cleavage-activating protein (SCAP) and HMG-CoA reductase (HMGR) both possess SSDs required for feedback regulatio...