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Analysis of the Borrelia burgdorferi GeHo fla gene and antigenic characterization of its gene product


, : Analysis of the Borrelia burgdorferi GeHo fla gene and antigenic characterization of its gene product. Journal of Bacteriology 173(4): 1452-1459

The fla gene of Borrelia burgdorferi GeHo was analyzed and expressed in Escherichia coli. The structural gene encodes a flagellar protein of 336 amino acids. Comparative sequence analysis of the amino acid sequence revealed a high degree of sequence conservation with flagellins from both phylogenetically related and unrelated bacteria. The antigenic properties of the B. burgdorferi Fla protein were studied by synthesizing overlapping octapeptides, which were screened by using a battery of different monoclonal and polyclonal antibodies from various species directed against native and denatured flagellar proteins. No single species-independent immunodominant epitope could be located. However, immunoreactive oligopeptides clustered within the variable middle region (N-180 to 1-260). This region could constitute a candidate antigen for more specific and sensitive serodiagnosis of Lyme borreliosis.

US$29.90

PMID: 1704884


Other references

Garrard, TA., 1974: The genus Haustator, subgenus Kurosioia in Australian waters (Turritellidae: Mollusca). Journal malac Soc Aust, 31: 15-17

Lalanne, P.; Chavel, P.; Taboury, J., 1989: Optical inner-product implementation of neural networks models. The Hopfield neural network model is described in terms of inner product, i.e., as a matched filtering step followed by a pattern synthesis step. Optical implementation by two cascaded coherent filtering setups with holographic matched filters is...

O.Callaghan D.J.; Cheevers W.P.; Gentry G.A.; Randall C.C., 1968: Kinetics of cellular and viral dna synthesis in equine abortion herpes virus infection of neopl l m cells inst chromatography. Virology 36(1): 104-114

Healey, F.P., 1970: The mechanism of hydrogen evolution by algae. Diss. Abstr.: 30B 3048

Alpha, S.R.; Brady, A.H., 1973: Optical activity and conformation of the cation carrier X537A. Journal of the American Chemical Society 95(21): 7043-7049

Reriani, M.K.; Flammer, A.J.; Jama, A.; Lerman, L.O.; Lerman, A., 2012: Novel functional risk factors for the prediction of cardiovascular events in vulnerable patients following acute coronary syndrome. Over the years there has been considerable improvement in the clinical outcomes of patients treated for acute coronary syndrome (ACS). Despite a significant reduction in acute mortality, a large percentage of patients post ACS continue to experien...

Kiddee, P.; Naidu, R.; Wong, M.H., 2013: Electronic waste management approaches: an overview. Electronic waste (e-waste) is one of the fastest-growing pollution problems worldwide given the presence if a variety of toxic substances which can contaminate the environment and threaten human health, if disposal protocols are not meticulously m...

Steponkus, P.L.; Lanphear, F., 1967: O. Refinement of the triphenyl tetrazolium chloride method of determining cold injury. The method of evaluating cold injury in woody plants by the use of triphenyl tetrazolium chloride had been refined to eliminate bias associated with visual differentiation between varying degrees of tetrazolium reduction and to predict tissue surv...

Kang JunKwon; Bae HyungSup; Kim YoungSuk; Cho KiHo; Lee KyungSup; Park EunKyung; Kim DongHyun, 2001: Anti-thrombosis of Chungpesagan-tang is activated by human intestinal bacteria. The possibility of Chungpesagan-tang, which has been recommended on the stroke patients with constipation in Korean traditional clinic, and its ingredients as a novel antithrombotic agent was evaluated. Most of its ingredients except Puerariae Rad...

Huet, C., 1965: Etude de la differentiation de l'appareil genital femelle de Tenebrio molitor (Coleoptere). Canc. Soc. Biol., Paris: 57-60