+ Resolve Article
+ Follow Us
Follow on FacebookFollow on Facebook
Follow on TwitterFollow on Twitter

+ Translate
+ Subscribe to Site Feed
GeoScience Most Shared ContentMost Shared Content

Changes related to the oestrous cycle in the expression of endometrial and oviductal proteins of mice

, : Changes related to the oestrous cycle in the expression of endometrial and oviductal proteins of mice. Journal of Reproduction and Fertility 95(1): 191-199

Soluble proteins extracted from the endometria and oviducts of normal sexually mature cycling Swiss Webster mice were analysed by two-dimensional high-resolution sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE). Thirty endometrial and 25 oviductal proteins showed differential expression related to the oestrous cycle. In the endometrium, 19 proteins were maximally expressed in the oestrous phase, and significantly decreased or could not be detected in dioestrus. Eleven additional proteins were more prominent in dioestrus. Most of these endometrial cyclic proteins were acidic. In the oviduct, almost two-thirds of cycle-related, differentially expressed proteins were more strongly expressed in dioestrus and were significantly less prominent or could not be detected in the oestrous phase. In contrast to the endometrial proteins, most of the oviductal cyclic proteins were basic. Fourteen proteins appeared to be identical in both organs, and five of these showed the same cyclic pattern of expression. The remaining cyclic proteins were organ specific and showed uterus- or oviduct-specific changes during the oestrous cycle. Among the cyclic proteins, four endometrial and two oviductal proteins were restricted to oestrus, whereas two endometrial and seven oviductal proteins were restricted to dioestrus. These proteins could serve as markers for specific phases of the oestrous cycle. Our data show that the mouse oestrous cycle is associated with consistent and predictable changes in protein expression in both the endometrium and oviduct.


PMID: 1625236

DOI: 10.1530/jrf.0.0950191

Other references

Barrett-Connor, E.; Connor, J.D., 1968: Shigellosis. Recent experience with 330 cases. Journal of the Florida Medical Association 55(6): 540-544

Flieger, S.; Boratynski, Z.; Welento, J.; Eustachiewicz, R.; Szalak, M.; Krzyzanowski, J.; Sawomirski, J., 1984: Location of nerve centres of the ovary and oviduct in the cow. Two cows were killed for histological examination of parts of the brain, spinal cord and autonomic ganglia 3 weeks after surgical removal of both ovaries and oviducts. The location of degenerative changes in nerve tissue suggested that motor nerve...

Seiffert, E., 1968: Clinical nutrition trials with Milasan, an unacidified prepared infant feed. Milasan contained 3.4% fat, 1.92% from milk fat and 1.48% from cottonseed oil, 2.1% protein, 7.68% carbohydrate, 0.49% minerals; 100 ml supplied 70 kcal, 190 IU vitamin A and 3 mg ascorbic acid. Of 146 infants given the preparation, 55.1% gained o...

Knapp, M.S., 1982: Urine specific gravity test strips. Lancet 2(8311): 1340-1340

Brooks, D.E.; Greiner, E.C.; Walsh, M.T., 1983: Conjunctivitis caused by Thelazia sp in a Senegal parrot. Thelazia sp. causing conjunctivitis in Senegal parrot, clinical report, removal of worm from eye by flushing after immobilizing worm with demecarium bromide

Garcia Estrada, C.; Romero Almaraz, M.D.L.urdes; Sanchez Hernandez, C., 2002: Comparison of rodent communities in sites with different degrees of disturbance in deciduous forest of southeastern Morelos, Mexico. This study is the first work that compares rodent communities in a deciduous forest in Mexico. It documents differences between sites experiencing different degrees of disturbance caused by firewood and lumber extraction, and cattle grazing; a rel...

Karimova, M.Kh.; Korneĭchuk, A.G.; Kubatiev, A.A.; Kudrin, V.S.; Mamysheva, O.D., 1994: A comparative study of the catecholamine content of the blood and urine in patients with facial paraspasm (Meige's syndrome). Catecholamines and DOPA excretion and televant levels in blood were measured in 17 patients with facial paraspasm and in 24 healthy subjects. The findings gave evidence for sympathetic-adrenal system adrenal activation and more intensive catechola...

Henrichs I.; Wolf A.; Musch K.; Teller W.M., 1982: Release of human placental lactogen during in vitro placental perfusion with and without somatostatin. Pediatric Research 16(10): 898

Nicol, A.Frederica.; Fernandes, A.Teresa.Gomes.; Bonecini-Almeida, M.da.Gloria., 2005: Immune response in cervical dysplasia induced by human papillomavirus: the influence of human immunodeficiency virus-1 co-infection -- review. Human immunodeficiency virus (HIV-1) has become an important risk factor for human papillomavirus (HPV) infection and the development of HPV associated lesions in the female genital tract. HIV-1 may also increase the oncogenicity of high risk HPV...

Loeber, G.; Dworkin, M.B.; Infante, A.; Ahorn, H., 1994: Characterization of cytosolic malic enzyme in human tumor cells. Cytosolic NADP+-dependent malic enzyme (ME) from human tumor cells was characterized in detail and compared to ME from normal human tissues produced in recombinant E. coli. Kinetic properties, size as seen in SDS gels, and HPLC elution profiles of...