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Chromatographic separation and partial identification of glycosidically bound volatile components of fruit


, : Chromatographic separation and partial identification of glycosidically bound volatile components of fruit. Journal Of Chromatography. 522: 255-266

Synthetic monoterpenic and aromatic ß-d-glucosides and ß-d-rutinosides were separated by Fractogel TSK HW-40 S chromatography according to their molecular size and interactions occurring between the aglycone moiety and the gel matrix. Under these conditions terpenyl rutinosides were eluted before the homologous glucoside derivatives. In the two classes, monoglucosides and rutinosides, aromatic glycosidically bound components had lower retention times than the corresponding terpenyl components. The use of over-pressure layer chromatography (OPLC) allowed the separation of glucoside and rutinoside derivatives, and in these two classes aromatic and several monoterpene compounds were well separated. Based on these results, the separation of glycosidically bound volatile components isolated from grapes (Muscat of Alexandria) a and apricot (Rouge du Roussillon) was undertaken using a three-step process. Silica gel fractionation allowed the separation of mono- and diglycosidic fractions present in apricot. Fifteen peaks were obtained from grape extracts by Fractogel chromatography, and under the same conditions sixteen and eleven peaks were detected in monosaccharidic and disaccharidic fractions, respectively isolated from apricot. The glycosidic fractions isolated by gel chromatography were purified by preparative OPLC, and some of them were sufficiently pure for a subsequent structural analysis. Moreover, partial identification of glycosidically bound volatile components separated by these two methods may be achieved using retention data when standard compounds are available; on the other hand, enzymatic hydrolysis of the isolated pure fractions gives information in the absence of reference compounds.

US$19.90

DOI: 10.1016/0021-9673(90)85195-2


Other references

Zapf, J., 1954: Vogelkundliche Mitteilungen. Ornithologische Beo-bachtungen in Gebiete des Glautales in den Jahren 1952 und 1953. Carinthia II. 1953; 143: 129-135

Tornek, A.; Field, T.; Hernandez-Reif, M.; Diego, M.; Jones, N., 2003: Music effects on EEG in intrusive and withdrawn mothers with depressive symptoms. THE EEG patterns of 48 intrusive and withdrawn mothers with depressive symptoms were assessed following a 20-minute music session to determine if the music had mood-altering effects. Half the mothers listened to classical music while half listened...

Sebestik, V.; Majsky, A.; Potmesilova, I.; Jelinek, J., 1976: The effect of splenectomy induced chemically by ethyl palmitate on the formation of hetero agglutinins in the rat. The formation of heteroagglutinins against human O-erythrocytes was pursued in Wistar rats chemically splenectomized by means of i.v. injections of ethyl palmitite. The initial inhibition in the formation of antibodies was replaced by a significan...

Vigny P., 1971: Fluorescence of nucleosides and nucleotides at room temperature. Comptes Rendus Hebdomadaires Des Seances De L'Academie Des Sciences Serie D Sciences Naturelles5: 3206-3209

Greenlaw, C.W.; Haug, M.T.; Geiger, G.S., 1981: Prediction of digoxin blood levels. American Journal of Hospital Pharmacy 38(2): 168, 170-168, 170

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Menaker, M.Z.mmerman, N., 1976: Role of the pineal in the circadian system of birds. American zoologist: er 16 (1) 45-55

Winid Bogumila, 2004: Seepage parameters in estimation of hydrogeological condition of salt deposit on the base of Wieliczka salt mine. Some seepage parameters are essential in the evaluation of the hydrogeological condition of the deposit. The base was analysis of chemical changes vs time and discharge changes vs time and detail analysis of forty choice seepages of Wieliczka Salt...

Thomas, L.H.; McDiarmid, A., 2004: Control of bovine TB. Veterinary Record 154(3): 96-96

Rohmann, I.; Larrain, C., 1973: Vascular involvement in Hodgkin's disease and reticulosarcoma. Revista Medica de Chile 101(7): 544-547