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Cryopreservation of Steinernema carpocapsae and Heterorhabditis bacteriophora

, : Cryopreservation of Steinernema carpocapsae and Heterorhabditis bacteriophora. Journal of Nematology 23(4): 432-437

A method for the cryopreservation of third-stage infective juveniles (IJ) of Steinernema carpocapsae and Heterorhabditis bacteriophora was developed. Cryoprotection was achieved by incubating the nematodes in 22% glycerol (S. carpocapsae) or 14% glycerol (H. bacteriophora) for 24 hours, followed by 70% methanol at 0 degrees C for 10 minutes. The viability of S. carpocapsae frozen in liquid nitrogen as 20 micro l volumes spread over cover slip glass was > 80%.


PMID: 19283150

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